HL60 Ast.4
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
human blood
packaging
tube of 5 μg 88120802-DNA-5UG
pkg of vial of cells 88120802-1VL
growth mode
Suspension
karyotype
2n = 48, hyperdiploid
morphology
Promyeloid, blast/promyelocyte
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Differentiation studies
Biochem/physiol Actions
Human Caucasian promyelocytic leukaemia
Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Do not allow the culture medium to become acidic.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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