生物来源
human blood
包装
tube of 5 μg 88120802-DNA-5UG
pkg of vial of cells 88120802-1VL
生长模式
Suspension
核型
2n = 48, hyperdiploid
形态学
Promyeloid, blast/promyelocyte
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Differentiation studies
生化/生理作用
Human Caucasian promyelocytic leukaemia
Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.
制备说明
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Do not allow the culture medium to become acidic.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
其他说明
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法规信息
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