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About This Item
UNSPSC Code:
41106514
Biological source:
rat testis
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 42
Morphology:
Epithelial
biological source
rat testis
packaging
tube of 5 μg 89031604-DNA-5UG, pkg of vial of cells 89031604-1VL
growth mode
Adherent
karyotype
2n = 42
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
A cell line from a spontaneous transplantable Leydig cell tumour in a Fischer strain rat, reported to secrete steroid, however over a 6 hour period the secretion of oestradiol and testosterone by radioimmunoassay could not be confirmed.
Rat Fischer Leydig cell testicular tumour
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
低风险生物材料
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