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Merck
CN

ED1

Reactivity Anti-macrophage, rat ), NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 89040701

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About This Item

UNSPSC Code:
41106514
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biological source

Mouse x Mouse Hybridoma

packaging

tube of 5 μg 89040701-DNA-5UG
pkg of vial of cells 89040701-1VL

ImmunoDon

BALB/c mouse spleen

Myeloma

Sp2/0

Reactivity

Anti-macrophage, rat

technique(s)

cell culture | mammalian: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−196°C

Immunogen

Highly purified recombinant human tumour necrosis factor alpha

Application

Not specified

Biochem/physiol Actions

Not specified
The antibody recognises a cytoplasmic antigen on monocytes and on most rat macrophage sub-populations. Please note that this hybridoma produces antibody in very low amounts (0.1 μg/ml).

Preparation Note

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C.
RPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS) (heat inactivated)

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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