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About This Item
UNSPSC Code:
41106514
Biological source:
human lung (fetal)
Growth mode:
Adherent
Karyotype:
2n = 46, diploid
Morphology:
Fibroblast
biological source
human lung (fetal)
packaging
tube of 5 μg 89071902-DNA-5UG, pkg of vial of cells 89071902-1VL
growth mode
Adherent
karyotype
2n = 46, diploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies
Biochem/physiol Actions
Derived from a 16-18 week old human foetus. Finite lifespan; capable of at least 57 population doublings.
Human foetal lung fibroblast
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 7,9
TPOX: 6,9
vWA: 17
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 7,9
TPOX: 6,9
vWA: 17
Preparation Note
Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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Regulatory Information
常规特殊物品
低风险生物材料
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