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Merck
CN

HFL1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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关于此项目

UNSPSC Code:
41106514
Biological source:
human lung (fetal)
Growth mode:
Adherent
Karyotype:
2n = 46, diploid
Morphology:
Fibroblast

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biological source

human lung (fetal)

packaging

tube of 5 μg 89071902-DNA-5UG, pkg of vial of cells 89071902-1VL

growth mode

Adherent

karyotype

2n = 46, diploid

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Virus studies

Biochem/physiol Actions

Derived from a 16-18 week old human foetus. Finite lifespan; capable of at least 57 population doublings.
Human foetal lung fibroblast
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 7,9
TPOX: 6,9
vWA: 17

Preparation Note

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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低风险生物材料
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