GH1

Hormone studies

Derived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1μg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.

Rat Wistar-Furth pituitary tumour

Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells b

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.