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About This Item
UNSPSC Code:
41106514
Biological source:
ovine brain
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Not specified
biological source
ovine brain
packaging
tube of 5 μg 89101302-DNA-5UG, pkg of vial of cells 89101302-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies: Visna virus
Biochem/physiol Actions
Initiated from the choroid plexus of an ovine brain. The cells support propagation of Visna virus, strain 1514 with resulting cytopathic effects. Tests have indicated that the cell line produces both detectable BVDV antigens and infectious BVDV.
Ovine brain choroid plexus
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
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