LL/2(LLc1)

Tumourigenicity, Metastasis and chemotherapeutic agent studies

Derived from the lung of a C57BL mouse implanted with a primary Lewis Lung carcinoma. The cells can be used as a model for metastasis and for studying the effects of chemotherapeutic agents.

Mouse C57BL Lewis lung carcinoma

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using mechanical technique (aspiration); 5% CO₂; 37°C. Cells detach as aggregates and are therefore difficult to count.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.