SV-T2
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
biological source
mouse embryo
packaging
tube of 5 μg 90030801-DNA-5UG
pkg of vial of cells 90030801-1VL
growth mode
Adherent
karyotype
2n = 40, modal No 41
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse BALB/3T3 clone A31
Cell Line Description
Derived by SV40 transformation of the line BALB/3T3 clone A31. The cells exhibit a high saturation density, lack contact inhibition and form tumours in appropriate hosts.
Application
Transformation, tumourigenicity and virus studies: SV40
Culture Medium
DMEM + 2mM Glutamine + 10% New Born Calf Serum (NBCS) or (5% NBCS + 5% Foetal Bovine Serum (FBS)).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1x1,000 to 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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