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Merck
CN

SV-T2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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关于此项目

UNSPSC Code:
41106514
Biological source:
mouse embryo
Growth mode:
Adherent
Karyotype:
2n = 40, modal No 41
Morphology:
Fibroblast

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biological source

mouse embryo

packaging

tube of 5 μg 90030801-DNA-5UG, pkg of vial of cells 90030801-1VL

growth mode

Adherent

karyotype

2n = 40, modal No 41

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Transformation, tumourigenicity and virus studies: SV40

Biochem/physiol Actions

Derived by SV40 transformation of the line BALB/3T3 clone A31. The cells exhibit a high saturation density, lack contact inhibition and form tumours in appropriate hosts.
Mouse BALB/3T3 clone A31

Preparation Note

DMEM + 2mM Glutamine + 10% New Born Calf Serum (NBCS) or (5% NBCS + 5% Foetal Bovine Serum (FBS)).
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1x1,000 to 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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