biological source
African green monkey kidney
packaging
tube of 5 μg 90032601-DNA-5UG, pkg of vial of cells 90032601-1VL
growth mode
Adherent
karyotype
2n = 60, pseudodiploid
morphology
Fibroblast
receptors
Not specified
Biochem/physiol Actions
Monkey African Green kidney fibroblast
The cells have been selected for sensitivity to SV40.
Preparation Note
DMEM (D6546) + 2 mM L-Glutamine (G7513) + 2% Tricine (1M solution at pH7.4) + 10% FBS / FCS (F2442).
Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.