FT
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
frog mouth
packaging
tube of 5 μg 90102525-DNA-5UG
pkg of vial of cells 90102525-1VL
growth mode
Adherent
karyotype
Modal no. 43
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Bull frog tongue
Derived from the tongue of a normal female bull frog. Incubation is optimal at 25°C but growth at a slower rate can continue even at 4°C where cells may be stored for prolonged periods.
Preparation Note
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Distilled H2O + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.02% EDTA at 10°C; 25°C. Capable of growth down to 4°C. Flask should not be gassed. Optimum pH is 8.0
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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