biological source
frog mouth
packaging
tube of 5 μg 90102525-DNA-5UG, pkg of vial of cells 90102525-1VL
growth mode
Adherent
karyotype
Modal no. 43
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Bull frog tongue
Derived from the tongue of a normal female bull frog. Incubation is optimal at 25°C but growth at a slower rate can continue even at 4°C where cells may be stored for prolonged periods.
Preparation Note
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Distilled H2O + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.02% EDTA at 10°C; 25°C. Capable of growth down to 4°C. Flask should not be gassed. Optimum pH is 8.0
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.