生物来源
frog mouth
包装
tube of 5 μg 90102525-DNA-5UG
pkg of vial of cells 90102525-1VL
生长模式
Adherent
核型
Modal no. 43
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
生化/生理作用
Bull frog tongue
Derived from the tongue of a normal female bull frog. Incubation is optimal at 25°C but growth at a slower rate can continue even at 4°C where cells may be stored for prolonged periods.
制备说明
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Distilled H2O + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.02% EDTA at 10°C; 25°C. Capable of growth down to 4°C. Flask should not be gassed. Optimum pH is 8.0
其他说明
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法规信息
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