biological source
human skin
packaging
tube of 5 μg 90110504-DNA-5UG
pkg of vial of cells 90110504-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Derived from a 6 year old Caucasian female with Gardner′s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Normal female karyotype.
Human Caucasian skin fibroblast Gardner′s syndrome
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 11,12
D5S818: 12
D7S820: 8,12
THO1: 6,9.3
TPOX: 10,12
vWA: 14,16
CSF1PO: 10,11
D13S317: 8,11
D16S539: 11,12
D5S818: 12
D7S820: 8,12
THO1: 6,9.3
TPOX: 10,12
vWA: 14,16
Preparation Note
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Monolayer should form in a 25cm2 in 3-4 days.
Other Notes
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