生物来源
human skin
包装
tube of 5 μg 90110504-DNA-5UG
pkg of vial of cells 90110504-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
Tumourigenicity studies
生化/生理作用
Derived from a 6 year old Caucasian female with Gardner′s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Normal female karyotype.
Human Caucasian skin fibroblast Gardner′s syndrome
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 11,12
D5S818: 12
D7S820: 8,12
THO1: 6,9.3
TPOX: 10,12
vWA: 14,16
CSF1PO: 10,11
D13S317: 8,11
D16S539: 11,12
D5S818: 12
D7S820: 8,12
THO1: 6,9.3
TPOX: 10,12
vWA: 14,16
制备说明
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Monolayer should form in a 25cm2 in 3-4 days.
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