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About This Item
UNSPSC Code:
41106514
Biological source:
mouse embryo
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast
biological source
mouse embryo
packaging
tube of 5 μg 90110523-DNA-5UG, pkg of vial of cells 90110523-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies.
Biochem/physiol Actions
Derived by exposing C3H/10T1/2 clone 8 cells with 3-methylcholanthrene for 24 hours. Subsequently they lost the property of contact inhibition. Inoculation of 100,000 cells or more into syngenic mice has been reported to produce fibrosarcoma.
Mouse C3H embryo
Preparation Note
BME (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
低风险生物材料
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