C3H/MCA clone 16
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
生物来源
mouse embryo
包装
tube of 5 μg 90110523-DNA-5UG
pkg of vial of cells 90110523-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
Tumourigenicity studies.
生化/生理作用
Derived by exposing C3H/10T1/2 clone 8 cells with 3-methylcholanthrene for 24 hours. Subsequently they lost the property of contact inhibition. Inoculation of 100,000 cells or more into syngenic mice has been reported to produce fibrosarcoma.
Mouse C3H embryo
制备说明
BME (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
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