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About This Item
UNSPSC Code:
41106514
Biological source:
raccoon uterus
Growth mode:
Adherent
Karyotype:
2n = 38
Morphology:
Fibroblast-like
biological source
raccoon uterus
packaging
tube of 5 μg 90111909-DNA-5UG, pkg of vial of cells 90111909-1VL
growth mode
Adherent
karyotype
2n = 38
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies: feline and canine viruses
Biochem/physiol Actions
Derived from trypsinised uterine tissue of an adult female Racoon. Pl.1Ut was developed for studies of feline and canine viruses since the Racoon is one of the few animals which is susceptible to both.
Racoon uterus
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm2 by washing 3 times with 0.255 trypsin or trypsin/EDTA; 5% CO2; 37°C. Finite lifespan; maximum 90 passages expected. Growth rate is very slow.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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