Detroit 525

Derived from a 3.5 year old Caucasian female who exhibited some of the symptoms diagnostic of Turners syndrome.

Human Skin Turners Syndrome

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,13
D5S818: 11,13
D7S820: 12
THO1: 9.3
TPOX: 8
vWA: 17,18

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.