SW 948

Tumourigenicity studies

Derived from a grade III adenocarcinoma of the colon of an 81 year old Caucasian female. The cells synthesise relatively large amounts of CEA (2,000ng/1,000,000 cells in 21 days).

Human Caucasian colon adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10,11
D16S539: 11,12
D5S818: 11
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,18

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; No CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.