生物来源
human colon
包装
tube of 5 μg 91030714-DNA-5UG
pkg of vial of cells 91030714-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Carcinoembryonic antigen (CEA)
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human Caucasian colon adenocarcinoma
细胞系描述
Derived from a grade III adenocarcinoma of the colon of an 81 year old Caucasian female. The cells synthesise relatively large amounts of CEA (2,000ng/1,000,000 cells in 21 days).
应用
Tumourigenicity studies
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10,11
D16S539: 11,12
D5S818: 11
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,18
CSF1PO: 12
D13S317: 10,11
D16S539: 11,12
D5S818: 11
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,18
培养基
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; No CO2; 37°C.
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