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About This Item
UNSPSC Code:
41106514
Biological source:
human liver
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Hyperdiploid to hypotriploid
Morphology:
Endothelial
biological source
human liver
packaging
tube of 5 μg 91091816-DNA-5UG, pkg of vial of cells 91091816-1VL
growth mode
Adherent
karyotype
Hyperdiploid to hypotriploid
morphology
Endothelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Derived from an ascites sample from a 52 year old male suffering from adenocarcinoma of the liver. The cells have now been shown to be endothelial in origin (In Vitro 1992;28A:136). The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Human liver adenocarcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 10,13
D7S820: 8,11
THO1: 7,9
TPOX: 9
vWA: 14,17
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 10,13
D7S820: 8,11
THO1: 7,9
TPOX: 9
vWA: 14,17
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
常规特殊物品
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