biological source
human thyroid
packaging
tube of 5 μg 92030502-DNA-5UG, pkg of vial of cells 92030502-1VL
growth mode
Adherent
karyotype
2n = 60-82, 82 predominant
morphology
Epithelial
products
Thyroglobulin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
A valuable model for the study of human follicular carcinoma.
Biochem/physiol Actions
Established from the metastases of a follicular carcinoma in a female patient. Tumorigenic in nude mice. The basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH). The cell line grows as a monolayer without the formation of follicles. Electronmicroscopy studies showed the presence of microvilli with interdigitations between neighbouring cells.
Human follicular thryoid carcinoma
Preparation Note
DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.