生物来源
human thyroid
包装
tube of 5 μg 92030502-DNA-5UG
pkg of vial of cells 92030502-1VL
生长模式
Adherent
核型
2n = 60-82, 82 predominant
形态学
Epithelial
产品
Thyroglobulin
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
metastasis
运输
dry ice
储存温度
−196°C
应用
A valuable model for the study of human follicular carcinoma.
生化/生理作用
Established from the metastases of a follicular carcinoma in a female patient. Tumorigenic in nude mice. The basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH). The cell line grows as a monolayer without the formation of follicles. Electronmicroscopy studies showed the presence of microvilli with interdigitations between neighbouring cells.
Human follicular thryoid carcinoma
制备说明
DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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