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ND7/23 Cell Line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92090903, Mouse x Rat Hybridoma nerve, Neuronal

Synonym(s):

ND7/23 Cells

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

Mouse x Rat Hybridoma nerve

packaging

tube of 5 μg 92090903-DNA-5UG
pkg of vial of cells 92090903-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Neuronal

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.
Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS
Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x104 viable cells/cm2 at 37°C and 5% CO2. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.

Biochem/physiol Actions

Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.
Mouse neuroblastoma x Rat neurone hybrid

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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