MOP-8
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
mouse embryo
packaging
tube of 5 μg 92111707-DNA-5UG
pkg of vial of cells 92111707-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Suitable host for transfection studies, polyoma virus replication studies
Biochem/physiol Actions
MOP-8 was developed by transforming NIH/3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus. The cells should be handled under laboratory containment level 2.
Mouse, SV40 Polyoma transformed NIH/3T3 cells
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA, 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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Certificates of Analysis (COA)
Lot/Batch Number
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