MOP-8

Suitable host for transfection studies, polyoma virus replication studies

MOP-8 was developed by transforming NIH/3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus. The cells should be handled under laboratory containment level 2.

Mouse, SV40 Polyoma transformed NIH/3T3 cells

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA, 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.