biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 93022521-DNA-5UG, pkg of vial of cells 93022521-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-IgG2a and IgG2b (allotype dependent), mouse
technique(s)
cell culture | mammalian: suitable
isotype
IgA
shipped in
dry ice
storage temp.
−196°C
Immunogen
A/PR/8/34 mouse pathogenic human influenza virus
Application
Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.
Biochem/physiol Actions
Mouse Sp2/0 x Mouse BALB/c spleen
The immunisation protocol involved repeated intranasal infection and a single i.v. injection before fusion. The antibody binds murine IgG2a of "a" and "b" allotype, Fc region. The isoallotype-specific Z34 IgA shows cross-reactivity with IgG2b ("b" allotype) of C57BL/6 origin but not of BALB/c origin. There is no cross-reaction with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.