Z34

A/PR/8/34 mouse pathogenic human influenza virus

Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.

Mouse Sp2/0 x Mouse BALB/c spleen

The immunisation protocol involved repeated intranasal infection and a single i.v. injection before fusion. The antibody binds murine IgG2a of "a" and "b" allotype, Fc region. The isoallotype-specific Z34 IgA shows cross-reactivity with IgG2b ("b" allotype) of C57BL/6 origin but not of BALB/c origin. There is no cross-reaction with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))

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