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UNSPSC Code:
41106514
Biological source:
Mouse x Mouse Hybridoma
Isotype:
IgA
Technique(s):
cell culture | mammalian: suitable
Myeloma:
Sp2/0
Reactivity:
Anti-IgG2a and IgG2b (allotype dependent), mouse
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 93022521-DNA-5UG, pkg of vial of cells 93022521-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-IgG2a and IgG2b (allotype dependent), mouse
technique(s)
cell culture | mammalian: suitable
isotype
IgA
shipped in
dry ice
storage temp.
−196°C
Immunogen
A/PR/8/34 mouse pathogenic human influenza virus
Application
Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.
Biochem/physiol Actions
Mouse Sp2/0 x Mouse BALB/c spleen
The immunisation protocol involved repeated intranasal infection and a single i.v. injection before fusion. The antibody binds murine IgG2a of "a" and "b" allotype, Fc region. The isoallotype-specific Z34 IgA shows cross-reactivity with IgG2b ("b" allotype) of C57BL/6 origin but not of BALB/c origin. There is no cross-reaction with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))
Other Notes
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法规信息
低风险生物材料
此项目有
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