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About This Item
UNSPSC Code:
41106514
Biological source:
human spleen
Growth mode:
Suspension
Karyotype:
Not specified
Morphology:
Lymphoblast
biological source
human spleen
packaging
tube of 5 μg 93031001-DNA-5UG, pkg of vial of cells 93031001-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Immortalising antigen-stimulated human B-lymphocytes by somatic hybridisation
Biochem/physiol Actions
A non Ig-secreting, HAT sensitive, 6-thioguanine resistant, human B lymphocyte. Derived from the parent line WIL2.NS (Sigma Catalogue number. 90112121).
Human Caucasian B lymphocyte
Preparation Note
Maintain cultures between 5x1,000-5x10,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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Regulatory Information
低风险生物材料
常规特殊物品
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