生物来源
human spleen
包装
tube of 5 μg 93031001-DNA-5UG
pkg of vial of cells 93031001-1VL
生长模式
Suspension
核型
Not specified
形态学
Lymphoblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Human Caucasian B lymphocyte
细胞系描述
A non Ig-secreting, HAT sensitive, 6-thioguanine resistant, human B lymphocyte. Derived from the parent line WIL2.NS (Sigma Catalogue number. 90112121).
应用
Immortalising antigen-stimulated human B-lymphocytes by somatic hybridisation
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 5x1,000-5x10,000 cells/ml; 5% CO2; 37°C.
其他说明
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