COLO 839

Tumourigenicity studies

Derived from the peripheral blood of a 43 year old Caucasian male with widespread melanoma. The cells were transformed with defective EBV (B-95-W8).
COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling.

Human Caucasian B lymphocyte

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,13
D5S818: 11,12
D7S820: 8,10
THO1: 9.3,10
TPOX: 8,11
vWA: 17,19

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.