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Merck
CN

COLO 720 L

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
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biological source

human blood

packaging

tube of 5 μg 93052623-DNA-5UG
pkg of vial of cells 93052623-1VL

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Biochem/physiol Actions

Human normal B lymphocytes
Normal B lymphoid cell line derived from the peripheral blood of a 74 year old Chinese female (the same patient as COLO 720E) with ovarian cancer. These B-lymphoid cells grow as characteristic clumps of cells in the media. Often the peripheral cells of the clumps have their spike-like uropods extending into the media.
STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 8,11
D16S539: 11
D5S818: 10,11
D7S820: 10
THO1: 9
TPOX: 11
vWA: 14

Preparation Note

Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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