biological source
human blood
packaging
tube of 5 μg 93052623-DNA-5UG, pkg of vial of cells 93052623-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human normal B lymphocytes
Normal B lymphoid cell line derived from the peripheral blood of a 74 year old Chinese female (the same patient as COLO 720E) with ovarian cancer. These B-lymphoid cells grow as characteristic clumps of cells in the media. Often the peripheral cells of the clumps have their spike-like uropods extending into the media.
STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 8,11
D16S539: 11
D5S818: 10,11
D7S820: 10
THO1: 9
TPOX: 11
vWA: 14
CSF1PO: 11,13
D13S317: 8,11
D16S539: 11
D5S818: 10,11
D7S820: 10
THO1: 9
TPOX: 11
vWA: 14
Preparation Note
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.