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About This Item
UNSPSC Code:
41106514
Biological source:
human brain
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 87-91, hypotetraploid
Morphology:
Glial
biological source
human brain
packaging
tube of 5 μg 93061119-DNA-5UG, pkg of vial of cells 93061119-1VL
growth mode
Adherent
karyotype
2n = 87-91, hypotetraploid
morphology
Glial
products
Not specified
receptors
Epidermal growth factor (EGF) R
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Derived from an adult female with glioblastoma multiforme treated with local brain irradiation and multi drug chemotherapy. By flow cytometry these cells were almost 100% positive for MHC Class I.
Human glioblastoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 12
D5S818: 12,13
D7S820: 11
THO1: 8
TPOX: 8
vWA: 15,16
CSF1PO: 10,11
D13S317: 9
D16S539: 12
D5S818: 12,13
D7S820: 11
THO1: 8
TPOX: 8
vWA: 15,16
Preparation Note
RPMI 1640 + 2mM Glutamine + 1% HT + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
常规特殊物品
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