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Merck
CN

DBTRG.05MG

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93061119, human brain, Glial

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UNSPSC Code:
41106514
Biological source:
human brain
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 87-91, hypotetraploid
Morphology:
Glial

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biological source

human brain

packaging

tube of 5 μg 93061119-DNA-5UG, pkg of vial of cells 93061119-1VL

growth mode

Adherent

karyotype

2n = 87-91, hypotetraploid

morphology

Glial

products

Not specified

receptors

Epidermal growth factor (EGF) R

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Tumourigenicity studies

Biochem/physiol Actions

Derived from an adult female with glioblastoma multiforme treated with local brain irradiation and multi drug chemotherapy. By flow cytometry these cells were almost 100% positive for MHC Class I.
Human glioblastoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 12
D5S818: 12,13
D7S820: 11
THO1: 8
TPOX: 8
vWA: 15,16

Preparation Note

RPMI 1640 + 2mM Glutamine + 1% HT + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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