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About This Item
UNSPSC Code:
41106514
Biological source:
Mouse x Mouse Hybridoma
Isotype:
IgG1, kappa
Technique(s):
cell culture | mammalian: suitable
Myeloma:
Sp2/0 Ag-14
Reactivity:
Anti-Madin-Darby canine kidney cells
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 93062219-DNA-5UG, pkg of vial of cells 93062219-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0 Ag-14
Reactivity
Anti-Madin-Darby canine kidney cells
technique(s)
cell culture | mammalian: suitable
isotype
IgG1, kappa
shipped in
dry ice
storage temp.
−196°C
Immunogen
Basolateral membrane fraction from dog intestine
Application
Study of cell surface polarity development
Biochem/physiol Actions
Mouse Sp2/0 Ag-14 x Mouse BALB/c spleen
The antibody is directed against a 58kDa protein present on the basolateral surface of Madin-Darby canine kidney cells of the MDCK cell line.
Preparation Note
EMEM(EBSS) + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.
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