FW 4.101

Sheep T cells

Detection of β1 integrin by FACS or immunohistology

Mouse Sp2/0 x Mouse BALB/c spleen

The antibody is directed against β1 integrin CD29 of many species including human, cattle and sheep, and shows an identical pattern of reactivity as other anti β1 integrin antibodies e.g. TS2/16.

IDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Following resuscitation the serum concentration should be increased to 20% and the tissue culture flask incubated in an upright position for 4-7 days until the doubling rate increases.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.