VPM 38

Ovine MHC class II

Quantification of DQ and DR expression on T cell subsets in adult and foetal peripheral blood, afferent lymph, lymph node and efferent lymph.

The antibody cross-reacts with bovine and human MHC class II. It reacts with MHC class II molecules on cell surfaces and on immunoblots. It reacts very weakly with ovine DQ L cell transfectants by immunofluorescence, but does not react with DQ blots and therefore is classified as strongly DR α specific, but weakly cross-reactive with DQ α.

Mouse NS0 x Mouse BALB/c spleen

Maintain cultures between 1-2x100,000 cells/ml; 5% CO₂; 37°C. Saturation density is reached at 3-9x100,000 cells/ml.

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.