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Merck
CN

BHK 21 CL13(IZS)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
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biological source

hamster kidney

packaging

tube of 5 μg 93120815-DNA-5UG
pkg of vial of cells 93120815-1VL

growth mode

Adherent

karyotype

2n = 44

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Application

Virus studies

Biochem/physiol Actions

Hamster Syrian kidney
Susceptible to a range of viruses including adenovirus 25, vaccinia, herpes simplex, reovirus 3, vesicular stomatitis, rubella, pseudorabies.

Preparation Note

GMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Regulatory Information

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