biological source
hamster kidney
packaging
tube of 5 μg 93120815-DNA-5UG, pkg of vial of cells 93120815-1VL
growth mode
Adherent
karyotype
2n = 44
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies
Biochem/physiol Actions
Hamster Syrian kidney
Susceptible to a range of viruses including adenovirus 25, vaccinia, herpes simplex, reovirus 3, vesicular stomatitis, rubella, pseudorabies.
Preparation Note
GMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
法规信息
低风险生物材料
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