biological source
human pancreas
description
Human primary pancreatic adenocarcinoma
packaging
tube of 5 μg 93120816-DNA-5UG
pkg of vial of cells 93120816-1VL
growth mode
Adherent
karyotype
2n = 59, near triploid
morphology
Epithelial
products
Mucin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human primary pancreatic adenocarcinoma
Cell Line Description
Derived from a 61 year old female with a primary adenocarcinoma of the pancreas. BxPC-3 cells produce mucin and the tumour produced in a nude mouse is moderately well to poorly differentiated like the primary adenocarcinoma.
Application
BXPC3 cell line has been used to determine the biological and therapeutic importance of gastrin gene expression in pancreatic adenocarcinomas.
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,11
D5S818: 11
D7S820: 10,13
THO1: 9
TPOX: 8
vWA: 14,18
CSF1PO: 13
D13S317: 11
D16S539: 9,11
D5S818: 11
D7S820: 10,13
THO1: 9
TPOX: 8
vWA: 14,18
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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