生物来源
human pancreas
描述
Human primary pancreatic adenocarcinoma
包装
tube of 5 μg 93120816-DNA-5UG
pkg of vial of cells 93120816-1VL
生长模式
Adherent
核型
2n = 59, near triploid
形态学
Epithelial
产品
Mucin
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human primary pancreatic adenocarcinoma
细胞系描述
Derived from a 61 year old female with a primary adenocarcinoma of the pancreas. BxPC-3 cells produce mucin and the tumour produced in a nude mouse is moderately well to poorly differentiated like the primary adenocarcinoma.
应用
BXPC3 cell line has been used to determine the biological and therapeutic importance of gastrin gene expression in pancreatic adenocarcinomas.
Tumourigenicity studies
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,11
D5S818: 11
D7S820: 10,13
THO1: 9
TPOX: 8
vWA: 14,18
CSF1PO: 13
D13S317: 11
D16S539: 9,11
D5S818: 11
D7S820: 10,13
THO1: 9
TPOX: 8
vWA: 14,18
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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