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RPMI 1846

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
Biological source:
hamster skin
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 44
Morphology:
Epithelial
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biological source

hamster skin

packaging

tube of 5 μg 93120834-DNA-5UG, pkg of vial of cells 93120834-1VL

growth mode

Adherent

karyotype

2n = 44

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Tumourigenicity studies

Biochem/physiol Actions

Derived from a spontaneous malignant melanoma in an aged golden Syrian hamster. The cells have the ability to produce melanin in vitro. The TD50 for new-born hamsters is 68 cells.
Hamster Syrian melanotic melanoma

Preparation Note

McCoy′s 5a + 2mM Glutamine + 10-20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 1.5x1,000,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.


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