biological source
Porcine thyroid
packaging
tube of 5 μg 93122323-DNA-5UG, pkg of vial of cells 93122323-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Thyroglobulin, plasminogen activator (u-PA)
receptors
Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Regulation of thyroid cell metabolism
Biochem/physiol Actions
Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.
Porcine thyroid epithelial
Preparation Note
Click:RPMI (1:1) + 10μg/ml Insulin + 10mM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 4 cells/cm2 using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.