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Merck
CN

PORTHOS

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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关于此项目

UNSPSC Code:
41106514
Biological source:
Porcine thyroid
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial

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biological source

Porcine thyroid

packaging

tube of 5 μg 93122323-DNA-5UG, pkg of vial of cells 93122323-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Thyroglobulin, plasminogen activator (u-PA)

receptors

Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Regulation of thyroid cell metabolism

Biochem/physiol Actions

Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.
Porcine thyroid epithelial

Preparation Note

Click:RPMI (1:1) + 10μg/ml Insulin + 10mM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 4 cells/cm2 using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

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