生物来源
Porcine thyroid
包装
tube of 5 μg 93122323-DNA-5UG
pkg of vial of cells 93122323-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Thyroglobulin, plasminogen activator (u-PA)
受体
Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
细胞系来源
Porcine thyroid epithelial
细胞系描述
Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.
应用
Regulation of thyroid cell metabolism
制备说明
Click:RPMI (1:1) + 10μg/ml Insulin + 10mM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 4 cells/cm2 using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO2; 37°C.
其他说明
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