MEG-01

Studies of physiological mechanisms governing the differentiation of human megakaryocyte and platelet release; the study of the biochemistry (and differentiation) of erythroid and myeloid cells.

Derived from the bone marrow of a 55 year old male with chronic myelogenous leukaemia. All karyotypes show the Philadelphia chromosome. Platelet GP IIb/IIIa antigen is expressed on more than 92% of cells. Cytoplasmic expression of GP Ib antigen was not detected on the surface of cells but was present in the cytoplasm 20% of cells. BA-1 antigen is expressed in almost all cells. FVIII-related antigen was not detected on the surface cells but was present in cytoplasm.

Human megakaryoblastic leukaemia

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 8
D16S539: 9
D5S818: 13
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subcultures are prepared by diluting the cell suspension into fresh medium at a ratio of 1:2 to 1:3. Cells should be seeded at 2-4 x 10⁵ cells/ml. Doubling time is 36-48 hours. 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.