MEG-01

Studies of physiological mechanisms governing the differentiation of human megakaryocyte and platelet release; the study of the biochemistry (and differentiation) of erythroid and myeloid cells.

Derived from the bone marrow of a 55 year old male with chronic myelogenous leukaemia. All karyotypes show the Philadelphia chromosome. Platelet GP IIb/IIIa antigen is expressed on more than 92% of cells. Cytoplasmic expression of GP Ib antigen was not detected on the surface of cells but was present in the cytoplasm 20% of cells. BA-1 antigen is expressed in almost all cells. FVIII-related antigen was not detected on the surface cells but was present in cytoplasm.

Human megakaryoblastic leukaemia

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 8
D16S539: 9
D5S818: 13
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subcultures are prepared by diluting the cell suspension into fresh medium at a ratio of 1:2 to 1:3. Cells should be seeded at 2-4 x 10⁵ cells/ml. Doubling time is 36-48 hours. 5% CO₂; 37°C.

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