DSL6A/C1

Study of pancreatic carcinomas, differentiation of acinar/ductal cells.

DSL6A/C1 were derived from a transplantable acinar cell carcinoma (DSL-6) that had been established from a primary carcinoma of the pancreas in an azaserine treated Lewis rat. The expression of the ductal cell marker OC.2 was shown with a sub-population expressing ductal marker OV-6, CK-7 and CK-19. DSL6A/C1 cells are reported to be tumorigenic in Lewis rats and produce solid tumours composed of duct like structures surrounded by dense fibrous tissue. The cell line lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and re-grafting.

Rat pancreatic carcinoma

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.